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maintenance medium mem  (Thermo Fisher)


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    Thermo Fisher maintenance medium mem
    Maintenance Medium Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maintenance medium mem/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    maintenance medium mem - by Bioz Stars, 2026-02
    90/100 stars

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    a, Western blot of iWAT lysate probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen protein in iWAT tissue of both lean and obese mice (bottom row), indicating successful Cre recombination and expression of hCD81-mNeonGreen recombinant protein in iWAT tissue. There is no significant difference between normalized amount of hCD81-mNeonGreen (using vinculin as a loading control) in iWAT between lean and obese groups. b, Western blot of lysate from left atria probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen of both lean and obese mice (bottom row), indicating presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen. c, Western blot of lysate from left ventricles probed with anti-mNeonGreen antibody (bottom row) demonstrates presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen in left ventricle (bottom row). Normalized amount of hCD81-mNeonGreen is significantly higher in left ventricles of obese compared to lean mice. (n = 3 lean mice and 3 obese mice, each with 2 technical replicates) *, p < 0.05, **, p < 0.01. d-f, Immunostaining of left ventricles of lean and obese mice demonstrated co-localization of troponin I, a <t>cardiomyocyte</t> marker ( d ), vimentin, a fibroblast marker ( e ), CD68, a macrophage marker ( f ), and mNeonGreen, indicating adipose tissue-specific EV deposition in these three cell types.
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    maintenance medium mem  (Thermo Fisher)
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    Thermo Fisher maintenance medium mem
    a, Western blot of iWAT lysate probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen protein in iWAT tissue of both lean and obese mice (bottom row), indicating successful Cre recombination and expression of hCD81-mNeonGreen recombinant protein in iWAT tissue. There is no significant difference between normalized amount of hCD81-mNeonGreen (using vinculin as a loading control) in iWAT between lean and obese groups. b, Western blot of lysate from left atria probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen of both lean and obese mice (bottom row), indicating presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen. c, Western blot of lysate from left ventricles probed with anti-mNeonGreen antibody (bottom row) demonstrates presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen in left ventricle (bottom row). Normalized amount of hCD81-mNeonGreen is significantly higher in left ventricles of obese compared to lean mice. (n = 3 lean mice and 3 obese mice, each with 2 technical replicates) *, p < 0.05, **, p < 0.01. d-f, Immunostaining of left ventricles of lean and obese mice demonstrated co-localization of troponin I, a <t>cardiomyocyte</t> marker ( d ), vimentin, a fibroblast marker ( e ), CD68, a macrophage marker ( f ), and mNeonGreen, indicating adipose tissue-specific EV deposition in these three cell types.
    Maintenance Medium Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a, Western blot of iWAT lysate probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen protein in iWAT tissue of both lean and obese mice (bottom row), indicating successful Cre recombination and expression of hCD81-mNeonGreen recombinant protein in iWAT tissue. There is no significant difference between normalized amount of hCD81-mNeonGreen (using vinculin as a loading control) in iWAT between lean and obese groups. b, Western blot of lysate from left atria probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen of both lean and obese mice (bottom row), indicating presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen. c, Western blot of lysate from left ventricles probed with anti-mNeonGreen antibody (bottom row) demonstrates presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen in left ventricle (bottom row). Normalized amount of hCD81-mNeonGreen is significantly higher in left ventricles of obese compared to lean mice. (n = 3 lean mice and 3 obese mice, each with 2 technical replicates) *, p < 0.05, **, p < 0.01. d-f, Immunostaining of left ventricles of lean and obese mice demonstrated co-localization of troponin I, a cardiomyocyte marker ( d ), vimentin, a fibroblast marker ( e ), CD68, a macrophage marker ( f ), and mNeonGreen, indicating adipose tissue-specific EV deposition in these three cell types.

    Journal: bioRxiv

    Article Title: A role of extracellular vesicle-mediated inter-organ communication in obesity-related arrhythmia

    doi: 10.1101/2025.09.13.676027

    Figure Lengend Snippet: a, Western blot of iWAT lysate probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen protein in iWAT tissue of both lean and obese mice (bottom row), indicating successful Cre recombination and expression of hCD81-mNeonGreen recombinant protein in iWAT tissue. There is no significant difference between normalized amount of hCD81-mNeonGreen (using vinculin as a loading control) in iWAT between lean and obese groups. b, Western blot of lysate from left atria probed with anti-mNeonGreen antibody demonstrates presence of hCD81-mNeonGreen of both lean and obese mice (bottom row), indicating presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen. c, Western blot of lysate from left ventricles probed with anti-mNeonGreen antibody (bottom row) demonstrates presence of adipose tissue-specific EVs tagged with hCD81-mNeonGreen in left ventricle (bottom row). Normalized amount of hCD81-mNeonGreen is significantly higher in left ventricles of obese compared to lean mice. (n = 3 lean mice and 3 obese mice, each with 2 technical replicates) *, p < 0.05, **, p < 0.01. d-f, Immunostaining of left ventricles of lean and obese mice demonstrated co-localization of troponin I, a cardiomyocyte marker ( d ), vimentin, a fibroblast marker ( e ), CD68, a macrophage marker ( f ), and mNeonGreen, indicating adipose tissue-specific EV deposition in these three cell types.

    Article Snippet: Cells were thawed and seeded on fibronectin-coated culture vessels and were maintained in cardiomyocyte maintenance basal medium (Axol Bioscience, cat. no. ax2530) as per the manufacturer’s instructions.

    Techniques: Western Blot, Expressing, Recombinant, Control, Immunostaining, Marker

    a, Representative action potential traces and quantification in atrial cardiomyocytes showing complete rescue of APD prolongation by Pyr3 treatment. b , The action potential morphology is restored to near-control levels, with normalized repolarization kinetics. (red, VAT EVs n = 6 wells; blue, VAT EVs + Pyr3, n = 6 wells) c-d, Similar analysis in ventricular cardiomyocytes demonstrating significant rescue of APD prolongation by TRPC3 inhibition (red, VAT EVs n = 19 wells; blue, VAT EVs + Pyr3, n = 5 wells) *, p < 0.05, ***, p < 0.001.

    Journal: bioRxiv

    Article Title: A role of extracellular vesicle-mediated inter-organ communication in obesity-related arrhythmia

    doi: 10.1101/2025.09.13.676027

    Figure Lengend Snippet: a, Representative action potential traces and quantification in atrial cardiomyocytes showing complete rescue of APD prolongation by Pyr3 treatment. b , The action potential morphology is restored to near-control levels, with normalized repolarization kinetics. (red, VAT EVs n = 6 wells; blue, VAT EVs + Pyr3, n = 6 wells) c-d, Similar analysis in ventricular cardiomyocytes demonstrating significant rescue of APD prolongation by TRPC3 inhibition (red, VAT EVs n = 19 wells; blue, VAT EVs + Pyr3, n = 5 wells) *, p < 0.05, ***, p < 0.001.

    Article Snippet: Cells were thawed and seeded on fibronectin-coated culture vessels and were maintained in cardiomyocyte maintenance basal medium (Axol Bioscience, cat. no. ax2530) as per the manufacturer’s instructions.

    Techniques: Control, Inhibition